Biocolor 可溶性胶原检测试剂盒
Biocolor Sircol Soluble Collagen Assay 

    采用与胶原特异性结合的Sircol染料，主要用于定量检测哺乳动物组织中抽提的酸溶性胶原和体外培养哺乳动物细胞释放到培养基中的胶原。

    溶于酸、胃蛋白等的可溶性胶原均可检测，具体可检测的胶原类型为I-V型，VI-XIV型胶原也可检测，但由于难以制备标准曲线，还无法定量。Sircol染料不能区分不同类型的胶原，但可与胶原的[Gly-X-Y]n螺旋结构特异性地结合。随着胶原变性为凝胶，该染料与胶原的结合力减弱。非脊椎动物与胶原由于变性温度较低、羟脯氨酸残基较少等原因，结合染料的能力较弱，需要制备种属特异性的纯化标准品，以绘制标准曲线。
  
实验步骤

1.绘制标准曲线
2.待测样本的制备：取50ul样本容易调整体积到100ul进行反应。根据反应的结果对样本浓度进行调整，使其在标准曲线范围内；
3.胶原—染料复合物的获得：加入1ml Sircol染料，混匀离心后，既得胶原—染料复合物，弃 上清，去掉未结合的染料
4.加入1ml 染料释放剂，将胶原—染料复合物中的染料释放出来
5.吸光度测定：用分光光度计540nm出定量测定。或使用colorimeter测定

产品订购 

    Product Code: S1000    110 assays 

    Product Code: S5000    440 assays

参考文献 
1
Anilkumar, T. V., Vineetha, V. P., Revi, D., Muhamed, J. and Rajan, A. (2014) Biomaterial properties of cholecystderived scaffold recovered by a non-detergent/enzymatic method. Journal of Biomedical Materials Research Part B:
Applied Biomaterials.
2
Arizmendi, N., Puttagunta, L., Chung, K. L., Davidson, C., Rey-Parra, J., Chao, D. V., Thebaud, B., Lacy, P. and
Vliagoftis, H. (2014) Rac2 is involved in bleomycin-induced lung inflammation leading to pulmonary fibrosis. Respiratory
research 15, 71.
3
Aschner, Y., Khalifah, A. P., Briones, N., Yamashita, C., Dolgonos, L., Young, S. K., Campbell, M. N., Riches, D. W.,
Redente, E. F., Janssen, W. J., et al. (2014) Protein Tyrosine Phosphatase α Mediates Profibrotic Signaling in Lung
Fibroblasts through TGF-β Responsiveness. The American journal of pathology 184, 1489–1502.
4
Banon-Maneus, E., Rovira, J., Ramirez-Bajo, M. J., Moya-Rull, D., Hierro-Garcia, N., Takenaka, S., Diekmann, F.,
Eickelberg, O., Königshoff, M. and Campistol, J. M. (2014) Wnt Pathway Activation in Long Term Remnant Rat Model.
BioMed Research International 2014.
5
Bellaye, P.-S., Burgy, O., Colas, J., Fabre, A., Marchal-Somme, J., Crestani, B., Kolb, M., Camus, P., Garrido, C. and
Bonniaud, P. (2014) Anti-fibrotic Role of αB-crystallin Inhibition in Pleural and Subpleural Fibrosis. American journal of
respiratory cell and molecular biology.
6
Bellaye, P.-S., Wettstein, G., Burgy, O., Besnard, V., Joannes, A., Colas, J., Causse, S., Marchal-Somme, J., Fabre, A.,
Crestani, B., et al. (2014) The small heat-shock protein αB-crystallin is essential for the nuclear localization of Smad4:
impact on pulmonary fibrosis. The Journal of pathology 232, 458–472.
7
Beyer, C., Zenzmaier, C., Palumbo-Zerr, K., Mancuso, R., Distler, A., Dees, C., Zerr, P., Huang, J., Maier, C.,
Pachowsky, M. L., et al. (2014) Stimulation of the soluble guanylate cyclase (sGC) inhibits fibrosis by blocking noncanonical TGFβ signalling. Annals of the rheumatic diseases annrheumdis–2013.
8
Biancheri, P., Di Sabatino, A., Ammoscato, F., Facciotti, F., Caprioli, F., Curciarello, R., Hoque, S. S., Ghanbari, A.,
Joe-Njoku, I., Giuffrida, P., et al. (2014) Absence of a role for interleukin-13 in inflammatory bowel disease. European
journal of immunology 44, 370–385.
9
Birukawa, N. K., Murase, K., Sato, Y., Kosaka, A., Yoneda, A., Nishita, H., Fujita, R., Nishimura, M., Ninomiya, T.,
Kajiwara, K., et al. (2014) Activated hepatic stellate cells are dependent on self collagen, cleaved by membrane type
1-matrix metalloproteinase for their growth. Journal of Biological Chemistry jbc–M113.
10
Bode, C., Kinjo, T., Alvord, W. G. and Klinman, D. M. (2014) Suppressive oligodeoxynucleotides reduce lung cancer
susceptibility in mice with silicosis. Carcinogenesis bgu005.
11
Chen, M.-H., Hu, C.-K., Chen, P.-R., Chen, Y.-S., Sun, J.-S. and Chen, M.-H. (2014) Dose-dependent regulation of cell
proliferation and collagen degradation by estradiol on ligamentum flavum. BMC musculoskeletal disorders 15, 238.12
Chen, T., Nie, H., Gao, X., Yang, J., Pu, J., Chen, Z., Cui, X., Wang, Y., Wang, H. and Jia, G. (2014) Epithelial–
mesenchymal transition involved in pulmonary fibrosis induced by multi-walled carbon nanotubes via TGF-beta/Smad
signaling pathway. Toxicology letters 226, 150–162.
13
Chen, Y.-S., Lee, S.-M., Lin, Y.-J., Chiang, S.-H. and Lin, C.-C. (2014) Effects of Danshensu and Salvianolic Acid B from
Salvia miltiorrhiza Bunge (Lamiaceae) on Cell Proliferation and Collagen and Melanin Production. Molecules 19, 2029–
2041.
14
Chen, Y.-P., Chen, W.-C., Wang, K.-C. and Chen, C.-H. (2014) Effectiveness of synovial fluid mesenchymal stem cells
embedded in alginate beads for treatment of steroid-induced avascular necrosis of the femoral head. Journal of
Orthopaedic Science 1–10.
15
Choi, E. M. (2014) Sunflower seed extract enhances the differentiation of osteoblastic MC3T3-E1 cells. Food and
Agricultural Immunology 25, 9–19.
16
Chung, C.-C., Hsu, R.-C., Kao, Y.-H., Liou, J.-P., Lu, Y.-Y. and Chen, Y.-J. (2014) Androgen Attenuates Cardiac
Fibroblasts Activations through Modulations of Transforming growth factor-β and Angiotensin II Signaling. International
Journal of Cardiology.
17
Conte, E., Iemmolo, M., Fagone, E., Gili, E., Fruciano, M., Genovese, T., Esposito, E., Cuzzocrea, S. and Vancheri, C.
(2014) Thymosin β4 reduces IL-17-producing cells and IL-17 expression, and protects lungs from damage in bleomycintreated mice. Immunobiology 219, 425–431.
18
Dakin, S. G., Smith, R. K. W., Heineg\a ard, D., Önnerfjord, P., Khabut, A. and Dudhia, J. (2014) Proteomic Analysis of
Tendon Extracellular Matrix Reveals Disease Stage-specific Fragmentation and Differential Cleavage of COMP (Cartilage
Oligomeric Matrix Protein). Journal of Biological Chemistry 289, 4919–4927.
19
Daryadel, A., Bogdanova, A., Gassmann, M., Mueller, X., Zünd, G., Seifert, B., Lehalle, C., Frossard, N. and Tavakoli,
R. (2014) Multikinase inhibitor sorafenib prevents pressure overload-induced left ventricular hypertrophy in rats by
blocking the c-Raf/ERK1/2 signaling pathway. Journal of Cardiothoracic Surgery 9, 81.
20
Den Hartog, G. J., Qi, S., van Tilburg, J. H., Koek, G. H. and Bast, A. (2014) Superoxide anion radicals activate hepatic
stellate cells after entry through chloride channels: A new target in liver fibrosis. European journal of pharmacology