Biocolor  细胞凋亡检测试剂盒 
Biocolor APOpercentage Assay
 
Biocolor 公司的专利产品
 
实验原理：哺乳动物细胞的细胞膜是磷脂双分子层结构。正常哺乳动物的细胞膜是卵磷脂和鞘磷脂朝向细胞的外侧，而磷脂酰乙醇胺和磷脂酰丝氨酸是朝向细胞的内侧，此时APOP染料不能进入细胞内。而当细胞发生凋亡时，磷脂酰丝氨酸就会翻转到细胞膜外侧，APOP染料会随着磷脂酰丝氨酸的翻转进入到细胞内，从而给凋亡细胞染色。

       卵磷脂和鞘磷脂    磷脂酰乙醇胺   磷脂酰丝氨酸     APOP染料
 
 

应用范围：适用于存在典型磷脂双分子层细胞膜的哺乳动物细胞，不适用于神经细胞，也不建议应用于非哺乳动物细胞。

检测仪器：倒置显微镜、酶标仪

试剂盒说明：该试剂盒中包含 APOPercentage 染料，能够特异性的与凋亡细胞结合，而不与坏死细胞结合，避免假阳性。通过倒置显微镜，可以观测并拍照被染色的凋亡细胞。同时，该试剂盒中还含有APOPercentage染料释放剂，能够使进入凋亡细胞中染料释放出来，从而用比色的方法进行定量。

 
产品订购 

Product Code: A1000    6 x 96 wells 或 4 x 24 wells

 

 

参考文献

 

1

Akpinar, A., Uğuz, A. C. and Nazıroğlu, M. (2014) Agomelatine and Duloxetine Synergistically Modulates Apoptotic

Pathway by Inhibiting Oxidative Stress Triggered Intracellular Calcium Entry in Neuronal PC12 Cells: Role of TRPM2 and

Voltage-Gated Calcium Channels. The Journal of membrane biology 247, 451–459.

2

ALKAHTANI, S. H. (2014) The Steroidal Na+/K+ ATPase Inhibitor 3-[(R)-3-Pyrrolidinyl] oxime Derivative (3-R-POD)

Induces Potent Pro-Apoptotic Responses in Colonic Tumor Cells. Anticancer Research 34, 2967–2971.

3

Argun, M., Tök, L., Uğuz, A. C., Çelik, Ö., Tök, Ö. Y. and Naziroğlu, M. (2014) Melatonin and amfenac modulate

calcium entry, apoptosis, and oxidative stress in ARPE-19 cell culture exposed to blue light irradiation (405 nm). Eye.

4

Beales, I. L., Garcia-Morales, C., Ogunwobi, O. O. and Mutungi, G. (2014) Adiponectin inhibits leptin-induced oncogenic

signalling in oesophageal cancer cells by activation of PTP1B. Molecular and cellular endocrinology 382, 150–158.

5

Cohen, M., Pierredon, S., Wuillemin, C., Delie, F. and Petignat, P. Acellular fraction of ovarian cancer ascites induce

apoptosis by activating JNK and inducing BRCA1, Fas and FasL expression in ovarian cancer cells.

6

Ghag, A. K., Gough, J. E. and Downes, S. (2014) The osteoblast and osteoclast responses to phosphonic acid containing

poly (ε-caprolactone) electrospun scaffolds. Biomaterials Science 2, 233–241.

7

Ghazizadeh, V. and Nazıroğlu, M. (2014) Electromagnetic radiation (Wi-Fi) and epilepsy induce calcium entry and

apoptosis through activation of TRPV1 channel in hippocampus and dorsal root ganglion of rats. Metabolic brain disease

1–13.

8

Jiang, X., Kanda, T., Nakamoto, S., Miyamura, T., Wu, S. and Yokosuka, O. (2014) Involvement of androgen receptor

and glucose-regulated protein 78kDa in human hepatocarcinogenesis. Experimental cell research 323, 326–336.

9

Kahya, M. C., Nazıroğlu, M. and Çiğ, B. (2014) Selenium Reduces Mobile Phone (900 MHz)-Induced Oxidative Stress,

Mitochondrial Function, and Apoptosis in Breast Cancer Cells. Biological trace element research 160, 285–293.

10

Lee, H.-S., Woo, S. J., Koh, H.-W., Ka, S.-O., Zhou, L., Jang, K. Y., Lim, H. S., Kim, H.-O., Lee, S.-I. and Park, B.-H.

(2014) Regulation of Apoptosis and Inflammatory Responses by Insulin-like Growth Factor Binding Protein 3 in

Fibroblast-like Synoviocytes and Experimental Animal Models of Rheumatoid Arthritis. Arthritis & Rheumatology 66,

863–873.

11

Nazıroğlu, M., Şahin, M., Çiğ, B., Aykur, M., Erturan, İ. and Ugan, Y. (2014) Hypericum perforatum Modulates Apoptosis

and Calcium Mobilization Through Voltage-Gated and TRPM2 Calcium Channels in Neutrophil of Patients with Behcet’s

Disease. The Journal of membrane biology 247, 253–262.

12

Nazıroğlu, M., Şenol, N., Ghazizadeh, V. and Yürüker, V. (2014) Neuroprotection Induced by N-acetylcysteine and

Selenium Against Traumatic Brain Injury-Induced Apoptosis and Calcium Entry in Hippocampus of Rat. Cellular and

molecular neurobiology 1–9.

13

Öhman, T., Teirilä, L., Lahesmaa-Korpinen, A.-M., Cypryk, W., Veckman, V., Saijo, S., Wolff, H., Hautaniemi, S.,

Nyman, T. A. and Matikainen, S. (2014) Dectin-1 Pathway Activates Robust Autophagy-Dependent Unconventional

Protein Secretion in Human Macrophages. The Journal of Immunology 192, 5952–5962.

14

Sagar, S., Esau, L., Moosa, B., Khashab, N. M., Bajic, V. B. and Kaur, M. (2014) Cytotoxicity and Apoptosis Induced by a

Plumbagin Derivative in Estrogen Positive MCF-7 Breast Cancer Cells. Anti-cancer agents in medicinal chemistry 14,

170.

15

SAIBU, M., SAGAR, S., GREEN, I., AMEER, F. and MEYER, M. (2014) Evaluating the Cytotoxic Effects of Novel Quinone

Compounds. Anticancer research 34, 4077–4086.

16

Song, M.-Y., Bae, U.-J., Jang, K. Y. and Park, B.-H. (2014) Transplantation of betacellulin-transduced islets improves

glucose intolerance in diabetic mice. Experimental & molecular medicine 46, e98.

17

Sund, J. and others. (2014) Proteomic Characterization of Biological Effects Induced by Engineered Nanomaterials.

18

Tök, L., Naziroglu, M., Uguz, A. C. and Tök, Ö. (2014) Elevated hydrostatic pressures induce apoptosis and oxidative

stress through mitochondrial membrane depolarization in PC12 neuronal cells: A cell culture model of glaucoma.

Journal of Receptors and Signal Transduction 1–7.

19

Venza, I., Visalli, M., Oteri, R., Teti, D. and Venza, M. (2014) Class I-specific histone deacetylase inhibitor MS-275

overrides TRAIL-resistance in melanoma cells by downregulating c-FLIP. International Immunopharmacology.

20

Witt, W., Büttner, P., Jannasch, A., Matschke, K. and Waldow, T. (2014) Reversal of myofibroblastic activation by

polyunsaturated fatty acids in valvular interstitial cells from aortic valves. Role of RhoA/G-actin/MRTF signalling.

Journal of molecular and cellular cardiology 74, 127–138.

21

Yamamoto, T., Takano, N., Ishiwata, K., Ohmura, M., Nagahata, Y., Matsuura, T., Kamata, A., Sakamoto, K.,

Nakanishi, T., Kubo, A., et al. (2014) Reduced methylation of PFKFB3 in cancer cells shunts glucose towards the

pentose phosphate pathway. Nature communications 5.