德国cellendes品牌的水凝胶三维细胞培养系统，与活体细胞外基质相似，采用该系统可使体外细胞培养更接近体内的生理特征，是基础研究、药物筛选和再生医学等领域细胞功能研究的理想选择。
水凝胶三维细胞培养产品介绍链接：http://www.qbioscience.com/productShow_537.html
cellendes水凝胶三维细胞培养产品常见问题如下：
1. 问：Cellendes 三维细胞水凝胶是否可以做切片？
答：由于凝胶的主要成分是水，所以做冷冻切片是很困难的，但可以根据实验的需求做石蜡切片。
Since hydrogels consist mostly of water, it is quite difficult to prepare frozen sections. I think the better way would be to prepare paraffin sections if this is compatible with the experimental analysis.
2. 问：水凝胶包埋的三维细胞是否可以用荧光染色，该如何操作？
答：水凝胶包埋的细胞是可以用小分子量的荧光试剂标记的。下面这篇protocol（MDCK cyst formation in 3-D Life Hydrogels）中介绍了如何用鬼笔环肽（phalloidin）标记肌动蛋白和Syto 24 Green标记细胞核。
下载链接：http://cellendes.com/images/pdfs/protocols/p_1_cyst_formation.pdf
如果您想免疫标记细胞，则比较困难了。因为大分子量的抗体穿透水凝胶会比小分子量的染料要缓慢的多，因此一抗二抗孵育时需要过夜孵育，且应尽量把3D细胞水凝胶做的薄一些，以便穿透间距小一些。如何将水凝胶做的薄一些，请跟技术人员索要protocol
cells imbedded in 3-D Life Hydrogels can be labelled with fluorescent small molecular reagents. An example how this is done using phalloidin to stain the actin network and Syto 24 Green to stain nuclei is illustrated in our Protocol No. 1 (MDCK cyst formation in 3-D Life Hydrogels),
If your customers would like to immuno-label cells in hydrogels, it will be a bit more difficult, because the high molecular weight of the antibody takes much longer to diffuse through the gel than small molecular staining reagents. In that case the incubations with the first and second antibody should be done overnight and the washing out of the antibody should be done during the following day with 3 washing steps. It is also important to make the gels as thin as possible, so the diffusion distance to reach the cells in the gel is not too far.
3. 问：客户培养的细胞是成骨细胞，请问推荐用哪个试剂盒？因为2D培养时需每天更换培养基，且更换的培养基是不同的，请问渗透进水凝胶的培养基能否被替换掉？
答：推荐的是3-D Life Dextran-CD Hydrogel Kit (Cat. No. G91-1) with RGD Peptide (Cat. No. 09-P-001)。
低分子量的物质渗透、穿透水凝胶是很快的，所以更换不同的培养基，水凝胶内的培养基能很快被替换。但有一些高分子量的成分，例如生长因子会渗透慢一些，可能会需要几个小时的时间。
A frequent medium exchange works well with 3-D Life hydrogels, because the diffusion of low molecular weight compounds is very rapid. The diffusion is slower, though, with higher molecular weight compounds, as for example growth factors. Depending on the molecular weight of the growth factor and the gel size it can take a while (up to hours) until the molecules have penetrated the entire gel.
4. 问：产品的效期是多次时间？
答：cellendes的产品效期是3年，我们备有库存，具体库存产品效期可查询。
5. 问：水凝胶试剂盒中的硫代甘油（Thioglycerol）是必须使用的吗？
答：在不使用RGD肽时，硫代甘油（Thioglycerol）不是必须品。之所以会在水凝胶试剂盒中放入硫代甘油（Thioglycerol），主要是因为有些老师会比较有无RGD肽的区别。在这种情况下，硫代甘油可以用来控制凝胶强度，以确保无RGD肽时与使用RGD肽时凝胶的强度一致。
The Thioglycerol is not necessary for cultivating chondrocytes without RGD. We include Thioglycerol in the kit in case someone wants to compare a gel with RGD to a gel without RGD. In this case Thioglycerol can be used to modify the control gel to make sure that the strength of this gel is comparable to the gel containing RGD.
问题会不断更新中，更多产品信息请联系：010-84504282 QQ：704852960
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